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{{Infobox Person |name= '''Louis Pasteur''' | image=Louis Pasteur.jpg | image_size = 180px| caption=French [microbiologist and [chemist | quotation=In the fields of observation, chance favors only the prepared mind. | birth_date={{birth date|1822|12|27|mf=y--> | birth_place=[Dole, Jura, [Jura (département), [France | dead=dead | death_date={{death date and age|1895|9|28|1822|12|27|mf=y--> | death_place=[Marnes-la-Coquette, [Hauts-de-Seine, [France -->Louis Pasteur (December 27 1822 – September 28 1895) was a France chemist best known for his remarkable breakthroughs in microbiology. His experiments confirmed the germ theory of disease, also reducing mortality from puerperal fever (childbed), and he created the first vaccine for rabies. He is best known to the general public for showing how to stop milk and wine from going sour - this process came to be called pasteurization. He is regarded as one of the three main founders of microbiology, together with Ferdinand Cohn and Robert Koch. He also made many discoveries in the field of chemistry, most notably the asymmetry of crystals.

Early life and biography Louis Jean Pasteur was born on December 27, 1822 in Dole, Jura in the Jura (département) region of France and grew up in the town of Arbois. There he later had his house and laboratory, which is a Pasteur museum today. His father, Jean Pasteur, was a poorly educated Tanning and a veteran of the Napoleonic wars. Louis's aptitude was recognized by his college headmaster, who recommended that the young man apply for the École Normale Supérieure, which accepted him. After serving briefly as professor of physics at Dijon Lycée in 1848, he became professor of chemistry at Strasbourg University, where he met and courted Marie Laurent, daughter of the university's rector in 1849. They were married on May 29, 1849 and together they had five children, only two of whom survived to adulthood. Throughout his whole life, Louis Pasteur remained an ardent Catholic. A well-known quotation illustrating this is attributed to him: "I have the faith of a Breton peasant, and by the time I die I hope to have the faith of a Breton peasant's wife."

Work on chirality and the polarization of light In Pasteur's early works as a chemist, he resolved a problem concerning the nature of tartaric acid (1849). A solution of this compound derived from living things (specifically, Lees (fermentation)) rotated the plane of polarization of light passing through it. The mystery was that tartaric acid derived by chemical synthesis had no such effect, even though its chemical reactions were identical and its elemental composition was the same.

Upon examination of the minuscule crystals of Sodium ammonium tartrate, Pasteur noticed that the crystals came in two asymmetric forms that were mirror images of one another. Tediously sorting the crystals by hand gave two forms of the compound: solutions of one form rotated polarized light clockwise, while the other form rotated light counterclockwise. An equal mix of the two had no polarizing effect on light. Pasteur correctly deduced the molecule in question was asymmetric and could exist in two different forms that resemble one another as would left- and right-hand gloves, and that the organic form of the compound consisted purely of the one type. This was the first time anyone had demonstrated chirality (chemistry) molecules.

Pasteur's doctoral thesis on crystallography attracted the attention of M. Puillet and he helped Pasteur garner a position of professor of chemistry at the Faculté (College) of Strasbourg.

In 1854, he was named Dean of the new College of Science in Lille. In 1856, he was made administrator and director of scientific studies of the École Normale Supérieure.

Germ theory Louis Pasteur demonstrated that the fermentation (food) process is caused by the growth of microorganisms, and that the growth of microorganisms in nutrient broths is not due to spontaneous generation.

He exposed boiled broths to air in vessels that contained a filter to prevent all particles from passing through to the growth medium, and even in vessels with no filter at all, with air being admitted via a long tortuous tube that would not allow dust particles to pass. Nothing grew in the broths; therefore, the living organisms that grew in such broths came from outside, as spores on dust, rather than spontaneously generated within the broth. This was one of the last and most important experiments disproving the theory of spontaneous generation. The experiment also supported germ theory.

While Pasteur was not the first to propose germ theory (Girolamo Fracastoro, Agostino Bassi, Friedrich Gustav Jakob Henle and others had suggested it earlier), he developed it and conducted experiments that clearly indicated its correctness and managed to convince most of Europe it was true. Today he is often regarded as the father of germ theory and bacteriology, together with Robert Koch.

Pasteur's research also showed that some microorganisms contaminated fermenting beverages. With this established, he invented a process in which liquids such as milk were heated to kill most bacteria and molds already present within them. He and Claude Bernard completed the first test on April 20, 1862. This process was soon afterwards known as pasteurisation (or "pasteurization" in America).

Beverage contamination led Pasteur to conclude that microorganisms infected animals and humans as well. He proposed preventing the entry of microorganisms into the human body, leading Joseph Lister to develop antiseptic methods in surgery.

In 1865, two parasitic diseases called pébrine and flacherie were killing great numbers of silkworms at Alès. Pasteur worked several years proving it was a microbe attacking silkworm eggs which caused the disease, and that eliminating this microbe within silkworm nurseries would eradicate the disease.

Pasteur also discovered anaerobe, whereby some microorganisms can develop and live without air or oxygen, called the Pasteur effect.

Immunology and Vaccination Pasteur's later work on diseases included work on chicken cholera. During this work, a culture of the responsible bacterium had spoiled and failed to induce the disease in some chickens he was infecting with the disease. Upon reusing these healthy chickens, Pasteur discovered that he could not infect them, even with fresh bacteria; the weakened bacteria had caused the chickens to become immune to the disease, even though they had only caused mild symptoms.

This discovery was serendipitous. His assistant Charles Chamberland (of French origin) had been instructed to inoculate the chickens after Pasteur went on holiday. Chamberland failed to do this, but instead went on holiday himself. On his return, the month old cultures made the chickens unwell, but instead of the infection being fatal, as it usually was, the chickens recovered completely. Chamberland assumed an error had been made, and wanted to discard the apparently faulty culture when Pasteur stopped him. Pasteur guessed the recovered animals now might be immune to the disease, as were the animals at Eure-et-Loir that had recovered from anthrax.

In the 1870s, he applied this immunisation method to anthrax, which affected cows, and aroused interest in combating other diseases.

in 1885.

Pasteur publicly claimed he had made the anthrax vaccine by exposing the bacillus to oxygen. His laboratory notebooks, now in the Bibliotheque Nationale in Paris, in fact show Pasteur used the method of rival Jean Joseph Henri Toussaint, a Toulouse veterinary surgeon, to create the anthrax vaccine. Adrien Loir, A l'ombre de Pasteur, éd. Le mouvement sanitaire, 1938, pp. 18 and 160. Pasteur by David V. Cohn, Ph.D., Emeritus of Biochemistry, University of Louisville Prof Cohn says : "Fortunately, Pasteur's colleagues Chamberlain* and Roux followed up the results of a research physician Jean-Joseph-Henri Toussaint who reported a year earlier that carbolic-acid/heated anthrax serum would immunize against anthrax. These results were difficult to reproduce and discarded although, as it turned out, Toussaint was on the right track. This led Pasteur and his assistants to substitute an anthrax vaccine prepared not dissimilar to that of Toussaint and different that Pasteur had announced". - Please, read : "Chamberland" instead of "Chamberlain". This method used the oxidizing agent potassium dichromate. Pasteur's oxygen method did eventually produce a vaccine but only after he had been awarded a patent on the production of an anthrax vaccine.

The notion of a weak form of a disease causing immunity to the virulent version was not new; this had been known for a long time for smallpox. Inoculation with smallpox was known to result in far less scarring, and greatly reduced mortality, in comparison to the naturally acquired disease. Edward Jenner had also discovered vaccination, using cowpox to give cross-immunity to smallpox (in 1796), and by Pasteur's time this had generally replaced the use of actual smallpox material in inoculation. The difference between smallpox vaccination and cholera and anthrax vaccination was that the weakened form of the latter two disease organisms had been generated artificially, and so a naturally weak form of the disease organism did not need to be found.

This discovery revolutionised work in infectious diseases, and Pasteur gave these artificially weakened diseases the generic name of vaccines, to honour Jenner's discovery. Pasteur produced the first vaccine for rabies by growing the virus in rabbits, and then weakening it by drying the affected nerve tissue.

The rabies vaccine was initially created by Emile Roux, a French doctor and a colleague of Pasteur who had been working with a killed vaccine produced by desiccating the spinal cords of infected rabbits. The vaccine had only been tested on eleven dogs before its first human trial.

This vaccine was first used on 9-year old Joseph Meister, on July 6, 1885, after the boy was badly mauled by a rabid dog. This was done at some personal risk for Pasteur, since he was not a licensed physician and could have faced prosecution for treating the boy. However, left without treatment, the boy faced almost certain death from rabies. After consulting with colleagues, Pasteur decided to go ahead with the treatment. The treatment proved to be a spectacular success, with Meister avoiding the disease; thus, Pasteur was hailed as a hero and the legal matter was not pursued. The treatment's success laid the foundations for the manufacture of many other vaccines. The first of the Pasteur Institutes was also built on the basis of this achievement.

Legal risk was not the only kind Pasteur undertook. In The Story of San Michele, Axel Munthe writes of the rabies vaccine research: Pasteur himself was absolutely fearless. Anxious to secure a sample of saliva straight from the jaws of a rabid dog, I once saw him with the glass tube held between his lips draw a few drops of the deadly saliva from the mouth of a rabid bull-dog, held on the table by two assistants, their hands protected by leather gloves.



Honors and final days Pasteur won the Leeuwenhoek medal, microbiology's highest honor, in 1895.

He was a Legion of Honor–one of only 75 in all of France.

He died in 1895, near Paris, from complications of a series of strokes that had started in 1868. He died while listening to the story of St Vincent de Paul, whom he admired and sought to emulate. Louis Pasteur - Catholic Encyclopedia He was buried in the Notre Dame de Paris, but his remains were reinterred in a crypt in the Institut Pasteur, Paris where he is remembered for his life-saving work.

Both Institut Pasteur and Université Louis Pasteur were named after him.

Pasteur was ranked #12 in the 1978 edition of Michael H. Hart's controversial book, The 100: A Ranking Of The Most Influential Persons in History. However, Pasteur was promoted to no. 11, replacing Karl Marx in the 1992 revised edition of the book.

Statements In his trumphal lecture at the Sorbonne in 1864 he said 'Never will the doctrine of spontaneous generation recover from the mortal blow struck by this simple experiment' Fox, Klaus Dose,'Molecular Evolution and The Origin of Live, W.H Freeman and Company, San Francisco, 1972, page4.171.' Alexander I. Oparin, Origin of Live, Dover Publications, NewYork, 1936, 1953 (reprent), p. 196.

See also

Notes References * Debré, P.; Forster, E.: Louis Pasteur. Johns Hopkins University Press, 1998; ISBN 0-8018-5808-9. A biography in English. * Gerald L. Geison, The private science of Louis Pasteur, Princeton University Press, 1995 (ISBN 0-691-03442-7). A historical review of Pasteur's work. * Tiner, John Hudson : "Louis Pasteur: Founder of Modern Medicine". Mott Media, 1990; ISBN 0-88062-159-1 (paperback). A biography.

* Latour, Bruno : "The Pasteurization of France". Harvard University Press, 1988; ISBN 0-674-65761-6 (paperback). A historical/sociological account.

External links

The complete work of Pasteur can be freely downloaded on site of BNF (Bibliothèque nationale de France, Gallica) (click on « Télécharger » (right, at the top)), with specific links:



Different articles published by Pasteur can be free downloaded on site of BNF (Bibliothèque nationale de France, Gallica) in the differents books of « Comptes rendus de l’Académie des sciences » Comptes rendus de l’Académie des sciences (free downloaded).



{{Persondata], Jura (département), France, [Hauts-de-Seine, Dole, Jura--> {{Infobox Person |name= '''Louis Pasteur''' | image=Louis Pasteur.jpg | image_size = 180px| caption=French [microbiologist and [chemist | quotation=In the fields of observation, chance favors only the prepared mind. | birth_date={{birth date|1822|12|27|mf=y--> | birth_place=[Dole, Jura, [Jura (département), [France | dead=dead | death_date={{death date and age|1895|9|28|1822|12|27|mf=y--> | death_place=[Marnes-la-Coquette, [Hauts-de-Seine, [France -->Louis Pasteur (December 27 1822 – September 28 1895) was a France chemist best known for his remarkable breakthroughs in microbiology. His experiments confirmed the germ theory of disease, also reducing mortality from puerperal fever (childbed), and he created the first vaccine for rabies. He is best known to the general public for showing how to stop milk and wine from going sour - this process came to be called pasteurization. He is regarded as one of the three main founders of microbiology, together with Ferdinand Cohn and Robert Koch. He also made many discoveries in the field of chemistry, most notably the asymmetry of crystals.

Early life and biography Louis Jean Pasteur was born on December 27, 1822 in Dole, Jura in the Jura (département) region of France and grew up in the town of Arbois. There he later had his house and laboratory, which is a Pasteur museum today. His father, Jean Pasteur, was a poorly educated Tanning and a veteran of the Napoleonic wars. Louis's aptitude was recognized by his college headmaster, who recommended that the young man apply for the École Normale Supérieure, which accepted him. After serving briefly as professor of physics at Dijon Lycée in 1848, he became professor of chemistry at Strasbourg University, where he met and courted Marie Laurent, daughter of the university's rector in 1849. They were married on May 29, 1849 and together they had five children, only two of whom survived to adulthood. Throughout his whole life, Louis Pasteur remained an ardent Catholic. A well-known quotation illustrating this is attributed to him: "I have the faith of a Breton peasant, and by the time I die I hope to have the faith of a Breton peasant's wife."

Work on chirality and the polarization of light In Pasteur's early works as a chemist, he resolved a problem concerning the nature of tartaric acid (1849). A solution of this compound derived from living things (specifically, Lees (fermentation)) rotated the plane of polarization of light passing through it. The mystery was that tartaric acid derived by chemical synthesis had no such effect, even though its chemical reactions were identical and its elemental composition was the same.

Upon examination of the minuscule crystals of Sodium ammonium tartrate, Pasteur noticed that the crystals came in two asymmetric forms that were mirror images of one another. Tediously sorting the crystals by hand gave two forms of the compound: solutions of one form rotated polarized light clockwise, while the other form rotated light counterclockwise. An equal mix of the two had no polarizing effect on light. Pasteur correctly deduced the molecule in question was asymmetric and could exist in two different forms that resemble one another as would left- and right-hand gloves, and that the organic form of the compound consisted purely of the one type. This was the first time anyone had demonstrated chirality (chemistry) molecules.

Pasteur's doctoral thesis on crystallography attracted the attention of M. Puillet and he helped Pasteur garner a position of professor of chemistry at the Faculté (College) of Strasbourg.

In 1854, he was named Dean of the new College of Science in Lille. In 1856, he was made administrator and director of scientific studies of the École Normale Supérieure.

Germ theory Louis Pasteur demonstrated that the fermentation (food) process is caused by the growth of microorganisms, and that the growth of microorganisms in nutrient broths is not due to spontaneous generation.

He exposed boiled broths to air in vessels that contained a filter to prevent all particles from passing through to the growth medium, and even in vessels with no filter at all, with air being admitted via a long tortuous tube that would not allow dust particles to pass. Nothing grew in the broths; therefore, the living organisms that grew in such broths came from outside, as spores on dust, rather than spontaneously generated within the broth. This was one of the last and most important experiments disproving the theory of spontaneous generation. The experiment also supported germ theory.

While Pasteur was not the first to propose germ theory (Girolamo Fracastoro, Agostino Bassi, Friedrich Gustav Jakob Henle and others had suggested it earlier), he developed it and conducted experiments that clearly indicated its correctness and managed to convince most of Europe it was true. Today he is often regarded as the father of germ theory and bacteriology, together with Robert Koch.

Pasteur's research also showed that some microorganisms contaminated fermenting beverages. With this established, he invented a process in which liquids such as milk were heated to kill most bacteria and molds already present within them. He and Claude Bernard completed the first test on April 20, 1862. This process was soon afterwards known as pasteurisation (or "pasteurization" in America).

Beverage contamination led Pasteur to conclude that microorganisms infected animals and humans as well. He proposed preventing the entry of microorganisms into the human body, leading Joseph Lister to develop antiseptic methods in surgery.

In 1865, two parasitic diseases called pébrine and flacherie were killing great numbers of silkworms at Alès. Pasteur worked several years proving it was a microbe attacking silkworm eggs which caused the disease, and that eliminating this microbe within silkworm nurseries would eradicate the disease.

Pasteur also discovered anaerobe, whereby some microorganisms can develop and live without air or oxygen, called the Pasteur effect.

Immunology and Vaccination Pasteur's later work on diseases included work on chicken cholera. During this work, a culture of the responsible bacterium had spoiled and failed to induce the disease in some chickens he was infecting with the disease. Upon reusing these healthy chickens, Pasteur discovered that he could not infect them, even with fresh bacteria; the weakened bacteria had caused the chickens to become immune to the disease, even though they had only caused mild symptoms.

This discovery was serendipitous. His assistant Charles Chamberland (of French origin) had been instructed to inoculate the chickens after Pasteur went on holiday. Chamberland failed to do this, but instead went on holiday himself. On his return, the month old cultures made the chickens unwell, but instead of the infection being fatal, as it usually was, the chickens recovered completely. Chamberland assumed an error had been made, and wanted to discard the apparently faulty culture when Pasteur stopped him. Pasteur guessed the recovered animals now might be immune to the disease, as were the animals at Eure-et-Loir that had recovered from anthrax.

In the 1870s, he applied this immunisation method to anthrax, which affected cows, and aroused interest in combating other diseases.

in 1885.

Pasteur publicly claimed he had made the anthrax vaccine by exposing the bacillus to oxygen. His laboratory notebooks, now in the Bibliotheque Nationale in Paris, in fact show Pasteur used the method of rival Jean Joseph Henri Toussaint, a Toulouse veterinary surgeon, to create the anthrax vaccine. Adrien Loir, A l'ombre de Pasteur, éd. Le mouvement sanitaire, 1938, pp. 18 and 160. Pasteur by David V. Cohn, Ph.D., Emeritus of Biochemistry, University of Louisville Prof Cohn says : "Fortunately, Pasteur's colleagues Chamberlain* and Roux followed up the results of a research physician Jean-Joseph-Henri Toussaint who reported a year earlier that carbolic-acid/heated anthrax serum would immunize against anthrax. These results were difficult to reproduce and discarded although, as it turned out, Toussaint was on the right track. This led Pasteur and his assistants to substitute an anthrax vaccine prepared not dissimilar to that of Toussaint and different that Pasteur had announced". - Please, read : "Chamberland" instead of "Chamberlain". This method used the oxidizing agent potassium dichromate. Pasteur's oxygen method did eventually produce a vaccine but only after he had been awarded a patent on the production of an anthrax vaccine.

The notion of a weak form of a disease causing immunity to the virulent version was not new; this had been known for a long time for smallpox. Inoculation with smallpox was known to result in far less scarring, and greatly reduced mortality, in comparison to the naturally acquired disease. Edward Jenner had also discovered vaccination, using cowpox to give cross-immunity to smallpox (in 1796), and by Pasteur's time this had generally replaced the use of actual smallpox material in inoculation. The difference between smallpox vaccination and cholera and anthrax vaccination was that the weakened form of the latter two disease organisms had been generated artificially, and so a naturally weak form of the disease organism did not need to be found.

This discovery revolutionised work in infectious diseases, and Pasteur gave these artificially weakened diseases the generic name of vaccines, to honour Jenner's discovery. Pasteur produced the first vaccine for rabies by growing the virus in rabbits, and then weakening it by drying the affected nerve tissue.

The rabies vaccine was initially created by Emile Roux, a French doctor and a colleague of Pasteur who had been working with a killed vaccine produced by desiccating the spinal cords of infected rabbits. The vaccine had only been tested on eleven dogs before its first human trial.

This vaccine was first used on 9-year old Joseph Meister, on July 6, 1885, after the boy was badly mauled by a rabid dog. This was done at some personal risk for Pasteur, since he was not a licensed physician and could have faced prosecution for treating the boy. However, left without treatment, the boy faced almost certain death from rabies. After consulting with colleagues, Pasteur decided to go ahead with the treatment. The treatment proved to be a spectacular success, with Meister avoiding the disease; thus, Pasteur was hailed as a hero and the legal matter was not pursued. The treatment's success laid the foundations for the manufacture of many other vaccines. The first of the Pasteur Institutes was also built on the basis of this achievement.

Legal risk was not the only kind Pasteur undertook. In The Story of San Michele, Axel Munthe writes of the rabies vaccine research: Pasteur himself was absolutely fearless. Anxious to secure a sample of saliva straight from the jaws of a rabid dog, I once saw him with the glass tube held between his lips draw a few drops of the deadly saliva from the mouth of a rabid bull-dog, held on the table by two assistants, their hands protected by leather gloves.



Honors and final days Pasteur won the Leeuwenhoek medal, microbiology's highest honor, in 1895.

He was a Legion of Honor–one of only 75 in all of France.

He died in 1895, near Paris, from complications of a series of strokes that had started in 1868. He died while listening to the story of St Vincent de Paul, whom he admired and sought to emulate. Louis Pasteur - Catholic Encyclopedia He was buried in the Notre Dame de Paris, but his remains were reinterred in a crypt in the Institut Pasteur, Paris where he is remembered for his life-saving work.

Both Institut Pasteur and Université Louis Pasteur were named after him.

Pasteur was ranked #12 in the 1978 edition of Michael H. Hart's controversial book, The 100: A Ranking Of The Most Influential Persons in History. However, Pasteur was promoted to no. 11, replacing Karl Marx in the 1992 revised edition of the book.

Statements In his trumphal lecture at the Sorbonne in 1864 he said 'Never will the doctrine of spontaneous generation recover from the mortal blow struck by this simple experiment' Fox, Klaus Dose,'Molecular Evolution and The Origin of Live, W.H Freeman and Company, San Francisco, 1972, page4.171.' Alexander I. Oparin, Origin of Live, Dover Publications, NewYork, 1936, 1953 (reprent), p. 196.

See also

Notes References * Debré, P.; Forster, E.: Louis Pasteur. Johns Hopkins University Press, 1998; ISBN 0-8018-5808-9. A biography in English. * Gerald L. Geison, The private science of Louis Pasteur, Princeton University Press, 1995 (ISBN 0-691-03442-7). A historical review of Pasteur's work. * Tiner, John Hudson : "Louis Pasteur: Founder of Modern Medicine". Mott Media, 1990; ISBN 0-88062-159-1 (paperback). A biography.

* Latour, Bruno : "The Pasteurization of France". Harvard University Press, 1988; ISBN 0-674-65761-6 (paperback). A historical/sociological account.

External links

The complete work of Pasteur can be freely downloaded on site of BNF (Bibliothèque nationale de France, Gallica) (click on « Télécharger » (right, at the top)), with specific links:



Different articles published by Pasteur can be free downloaded on site of BNF (Bibliothèque nationale de France, Gallica) in the differents books of « Comptes rendus de l’Académie des sciences » Comptes rendus de l’Académie des sciences (free downloaded).



{{Persondata], Jura (département), France, [Hauts-de-Seine, Dole, Jura-->

Louis Pasteur
Introduction Although regarded as one of the greatest scientists in history, Louis Pasteur was not a particularly good student. He did, however, show an aptitude for mathematics ...

BBC - History - Louis Pasteur (1822 - 1895)
Pasteur was a French chemist and biologist who proved the germ theory of disease and invented the process of pasteurisation.

Louis Pasteur
Louis Pasteur with tribute paid to him as a benefactor of humanity. The Welcome Institute, London.

Louis Pasteur::
Louis Pasteur was born in 1822 in Dole, France. Louis Pasteur’s name is forever cemented in the history of medicine. He, along with Alexander Fleming, Edward Jenner, Robert Koch ...

Louis Pasteur - Wikipedia, the free encyclopedia
Louis Pasteur (27 December 1822 – 28 September 1895),a French chemist and microbiologist, is best known for his remarkable breakthroughs in the causes and prevention of disease.

Site Web de la Villa Louis Pasteur
Le site Web de la Villa Louis Pasteur. Accueil et logement d'enseignants chercheurs

Louis Pasteur. by Brig Klyce
The greatest biologist of the nineteenth century was Louis Pasteur (1822-1895). His work had both practical use and profound theoretical significance.

Site Web de la Villa Louis Pasteur

Louis Pasteur
Louis Pasteur was a French Scientist. He was not a doctor, or even a medical scientist. He became known throughout ...

Pasteur, Louis (1822-1895) -- from Eric Weisstein's World of ...
French chemist who studied the effect of tartaric acid on polarized light. Under a microscope in 1848, he discovered that there were two types of tartrate crystals which were ...

 

Louis Pasteur



 
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